The exercise introduces the FIA (Flow Injection Analysis) technique, in the way that the participant can familiarize themselves with all essential operations details corresponding to FIA system. Understand the "mechanism" of FIA technique and the most important conditions of the FIA experiment can have a dramatic impact on the FIA experimental results.
FIA was first described by Ruzicka and Hansen in Denmark and Stewart and coworkers in United States in the middle of 1970s1,2. Flow-injection method is "an automatic assay on the basis of the number of samples alone"3, which allows performing of the analysis involving low volumes of sample and reagents leading to better sensitivity, but without any loss of reproducibility in performing the operation. Chemical procedures, which are not reliable manually (e. g. the reaction can not reach the equilibrium, the reaction product is not stable in time, etc) can be successfully released with FIA. The advantages mentioned above already turned FIA into a powerful technique with wide applications in different field (e. g. medicine, agriculture, environmental, etc).
The proposed exercise is divided in two parts: the first part deals with "practical" details of preparing the FIA system before analysis and the latter is the spectrophotometric determination of cuppersurfate using FIA.
1. J. Ruzicka, E. H. Hansen, Anal. Chim. Acta, 1975, 78, 145.
2. K. K. Stewart, G. R. Beecher, P. E. Hare, Anal. Biochem. 1976, 70, 167.
3. J. Ruzicka, E. H. Hansen, Flow Injection Analysis, edit. John Wiley & Sons, 1981, New York, USA.